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Merck KGaA
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AcceGen Biotechnology
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Image Search Results
Journal: bioRxiv
Article Title: ImplantoMetrics - Multidimensional trophoblast invasion assessment by combining 3D- in-vitro modeling and deep learning analysis
doi: 10.1101/2025.04.25.650556
Figure Lengend Snippet: A) On day one, HESC cells were seeded into a 96-well plate. On day two, a Matrigel layer was applied and HEC-1-A cells were added on top. In parallel, BLSs were grown from Sw.71 cells in a separate ultra-low attachment 96-well plate. After two days, the BLS developed a spherical structure. On day 4, the BLS was transferred to the cell layer model, placing the BLS in the center of the wells. BLS invasion was monitored for up to six days. During that period, the effect of different drugs on BLS invasion can be tested. B) ImplantoMetrics was used to analyze the invasion process and it gives six parameters that are important in the assessment of invasion success: spheroid radius, total spheroid and cell projections area, migration/invasion radius, number of cell projections, distribution of migration/invasion, and circularity. Each parameter has a different level of significance in implantation assessment and influences the process differently over time.
Article Snippet: Cell lines: Sw.71 ,
Techniques: Migration
Journal: bioRxiv
Article Title: ImplantoMetrics - Multidimensional trophoblast invasion assessment by combining 3D- in-vitro modeling and deep learning analysis
doi: 10.1101/2025.04.25.650556
Figure Lengend Snippet: The mean Invasion factor (σIF) (n=3, distinct samples) at three different indomethacin concentrations (0.2, 0.4, and 0.6 μg/ml) is shown for three different in vitro models, measured every 8 hours with the Cytation 7: A) combined endometrial model (HESC with Matrigel and HEC-1-A), B) HESC with Matrigel, and C) HEC-1-A with Matrigel. Error bars represent the standard deviation (± SD). Asterisks indicate significant differences (Welch’s t-test, one-sided) between untreated controls and indomethacin treated cells.
Article Snippet: Cell lines: Sw.71 ,
Techniques: In Vitro, Standard Deviation